To investigate the antioxidant effects of Isunsoyojoyoungtang(I.S.J.T), we administrated acetaminophen(500 ㎎/㎏, i.p.) that induced hepatic lipid peroxidation to starved mice for 24hrs after pretreatment of I.S.J.T for 6 days. And the effects of I.S.J.T on the hepatic enzymes(glutathion-S-transferase, superoxide dismutase, glutathion peroxidase and catala se) which participate in lipid peroxidation and glutathion metabolism were investigated.
The results were as follows
1. GST(glutathion-S-transferase) activity in the acetaminphen treated group was remarkably reduced compared with saline treated group (control group). However, in the pretreated group of I.S.J.T for 6 days GST activity was increased about, 1.6 fold.
2. Compared with control group, SOD(superoxide dismutase) activity was decreased by 26.9% in the acetaminophen treated group. On the other hand, in the I.S.J.T pretreated group SOD activity was almost recovered to the level of control group.
3. In the acetaminophen treated group, GSH-Px(glutathion peroxidase) activity was decreased by 47.1% compare with control. group. And in the I.S.J.T pretreated group, GSH-Px activity was slightly increased.
4. Catalase activity was also decreased by 23.8% in actaminophen treated group. However, in the I.S.J.T pretreated group, catalase activity was increased above 2 fold compared with control group.
5. In the acetaminophen treated group, malondialdehyde values of plasma and cytosolic fraction were significantly increased compared with control group. However, after the I.S.J.T treated group, lipid peroxide formation was decreased by 54% in plasma, and remarkably decreased 3.4 fold in cytosolic fraction.
These results suggest that I.S.J.T plays a protective role in the formation of acetaminophen induced lipid peroxidation.
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